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Major amounts of inhibitory activity were detected in GM-17 and CM after 8 and 12 h of growth, respectively. Thus, for purification purposes, 1 liter of 10-h CM culture (with a total of 100,000 AU) was used due to its optimum production…

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It has recently become possible to experimentally measure the effects of all amino-acid point mutations to proteins using deep mutational scanning. These experimental measurements can inform site-specific phylogenetic substitution models of…